产品描述

The human macrophage inflammatory protein-3α (MIP-3α) ELISA Kit is used to quantitatively measure human MIP-3α concentrations in serum, plasma, or cell culture supernates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of MIP-3α in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

MIP-3α, also called CCL20, is primarily expressed in the liver, colon, prostate, cervix, and skin. CCL20 only binds to CCR6 and is involved in numerous diseases, including rheumatoid arthritis and human immunodeficiency virus infection. CCL20-CCR6 axis also plays a key role in tumor progression. In addition, CCL20 can also directly mediate the migration and/or invasion process of various cancer cells, including lung adenocarcinoma, cholangiocarcinoma, colon cancer, and HCC. CCL20 can also attract tumor-promoting immune-suppressive cells to the tumor microenvironment (TME), which may contribute to the immune-evasive potential of the tumor and tumor progression.

别名

Beta chemokine exodus 1 ELISA Kit; Beta-chemokine exodus-1 ELISA Kit; C C motif chemokine ligand 20 ELISA Kit; C-C motif chemokine 20 ELISA Kit; CC chemokine LARC ELISA Kit; Ccl20 ELISA Kit; CCL20(2-70) ELISA Kit; CCL20_HUMAN ELISA Kit; Chemokine (C C motif) ligand 20 ELISA Kit; Chemokine C-C motif chemokine 20 ELISA Kit; Chemokine CC motif ligand 20 ELISA Kit; CKb4 ELISA Kit; Exodus 1 ELISA Kit; Exodus ELISA Kit; LARC ELISA Kit; Liver and activation regulated chemokine ELISA Kit; Liver and activation-regulated chemokine ELISA Kit; Macrophage inflammatory protein 3 alpha ELISA Kit; MIP 3 alpha ELISA Kit; MIP 3A ELISA Kit; MIP-3-alpha ELISA Kit; MIP-3a ELISA Kit; MIP3 alpha ELISA Kit; MIP3A ELISA Kit; SCYA20 ELISA Kit; Small inducible cytokine A20 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 20 ELISA Kit; Small-inducible cytokine A20 ELISA Kit; ST38 ELISA Kit

缩写

CCL20

Uniprot No

P78556

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-3α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		85-96
1:2		Average %		94
		Range %		87-101
1:4		Average %		99
		Range %		96-104
1:8		Average %		95
		Range %		92-99

回收率

The recovery of human MIP-3α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		85-99
EDTA plasma (n=4)		96		92-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.092 2.100 2.096 1.994 500 1.665 1.765 1.715 1.613 250 1.269 1.288 1.279 1.177 125 0.775 0.825 0.800 0.698 62.5 0.418 0.399 0.409 0.307 31.2 0.324 0.304 0.314 0.212 15.6 0.215 0.201 0.208 0.106 0 0.106 0.098 0.102

货期

3-5 working days