产品描述
AMBP (Alpha-1-Microglobulin/Bikunin Precursor)是一种由肝脏合成的蛋白质,在尿液和血浆中都有存在。其主要功能包括:抑制蛋白酶的活性、调节细胞生长、促进血管生成和抑制肿瘤的生长等。
华美生物所提供的Human alpha-1-microglobulin/bikunin precursor,AMBP ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、尿液、组织匀浆样本中的AMBP,其灵敏度为0.039 ng/mL,检测范围为0.156 ng/mL-10 ng/mL。
别名
A1M ELISA Kit; Alpha 1 microglobulin/bikunin precursor ELISA Kit; Alpha 1 microglycoprotein ELISA Kit; Alpha-1 microglycoprotein ELISA Kit; Alpha-1-microglobulin ELISA Kit; AMBP ELISA Kit; AMBP_HUMAN ELISA Kit; Bikunin ELISA Kit; Complex-forming glycoprotein heterogeneous in charge ELISA Kit; EDC1 ELISA Kit; Growth inhibiting protein 19 ELISA Kit; HCP ELISA Kit; HI-30 ELISA Kit; HI30 ELISA Kit; IATIL ELISA Kit; ITI ELISA Kit; ITI-LC ELISA Kit; ITIL ELISA Kit; Microglobulin (alpha 1) ELISA Kit; Protein AMBP ELISA Kit; Protein HC ELISA Kit; Trypstatin ELISA Kit; Uristatin ELISA Kit; Uronic-acid-rich protein ELISA Kit; UTI ELISA Kit
缩写
AMBP
Uniprot No
P02760
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
91 |
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| Range % |
86-98 |
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| 1:2000 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:4000 |
Average % |
85 |
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| Range % |
81-88 |
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| 1:8000 |
Average % |
89 |
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| Range % |
83-96 |
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回收率
| The recovery of human AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
87-102 |
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| EDTA plasma (n=4) |
96 |
91-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.862 |
2.793 |
2.828 |
2.679 |
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| 5 |
2.531 |
2.567 |
2.549 |
2.400 |
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| 2.5 |
2.002 |
2.054 |
2.028 |
1.879 |
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| 1.25 |
1.296 |
1.315 |
1.306 |
1.157 |
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| 0.625 |
0.643 |
0.672 |
0.658 |
0.509 |
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| 0.312 |
0.441 |
0.453 |
0.447 |
0.298 |
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| 0.156 |
0.292 |
0.300 |
0.296 |
0.147 |
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| 0 |
0.147 |
0.151 |
0.149 |
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货期
3-5 working days