产品描述
The Rat Apolipoprotein A-IV (APOA4) ELISA kit is a powerful tool for the quantitative detection of Apolipoprotein A-IV in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat) samples.
This ELISA kit offers a wide detection range of 9.38 ng/mL to 600 ng/mL and a sensitivity of 2.34 ng/mL, enabling precise and accurate quantification of APOA4 in your samples. The assay time is short, ranging from 1 to 5 hours, and requires only a small sample volume of 50-100ul.
The assay principle is a sandwich method, where the APOA4 in the sample is captured by a coated antibody and detected by a biotinylated detection antibody, followed by a streptavidin-HRP conjugate, and finally substrate solution. The signal is detected at a wavelength of 450 nm.
This kit is suitable for research in various areas and is particularly useful for studies involving cancer and metabolism research. The Rat Apolipoprotein A-IV (APOA4) ELISA kit provides reliable and consistent results for your experiments.
别名
Apoa4Apolipoprotein A-IV ELISA kit; Apo-AIV ELISA kit; ApoA-IV ELISA kit; Apolipoprotein A4 ELISA kit
缩写
APOA4
Uniprot No
P02651
灵敏度
2.34 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat APOA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:200 | Average % | 93 | |
| Range % | 87-97 | |
| 1:400 | Average % | 101 | |
| Range % | 97-105 | |
| 1:800 | Average % | 92 | |
| Range % | 88-96 | |
| 1:1600 | Average % | 95 | |
| Range % | 91-101 | |
回收率
| The recovery of rat APOA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 89 | 83-94 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 600 | 2.567 | 2.615 | 2.591 | 2.454 | | | 300 | 1.934 | 2.078 | 2.006 | 1.869 | | | 150 | 1.324 | 1.397 | 1.361 | 1.224 | | | 75 | 0.831 | 0.822 | 0.827 | 0.690 | | | 37.5 | 0.581 | 0.574 | 0.578 | 0.441 | | | 18.75 | 0.334 | 0.323 | 0.329 | 0.192 | | | 9.38 | 0.261 | 0.268 | 0.265 | 0.128 | | | 0 | 0.136 | 0.137 | 0.137 | | |
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货期
3-5 working days
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