产品描述
This Rat Aβ1-42 ELISA Kit was designed for the quantitative measurement of Rat Aβ1-42 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 pg/mL-60 pg/mL and the sensitivity is 0.225 pg/mL .
别名
Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
缩写
Aβ1-42
灵敏度
0.225 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 83-90 | |
| 1:2 | Average % | 94 | |
| Range % | 90-98 | |
| 1:4 | Average % | 101 | |
| Range % | 97-104 | |
| 1:8 | Average % | 96 | |
| Range % | 93-99 | |
回收率
| The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 100 | 95-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 60 | 2.281 | 2.274 | 2.278 | 2.136 | | | 30 | 1.599 | 1.576 | 1.588 | 1.446 | | | 15 | 0.967 | 0.924 | 0.946 | 0.804 | | | 7.5 | 0.538 | 0.528 | 0.533 | 0.391 | | | 3.75 | 0.395 | 0.371 | 0.383 | 0.241 | | | 1.8 | 0.321 | 0.312 | 0.317 | 0.175 | | | 0.9 | 0.235 | 0.250 | 0.243 | 0.101 | | | 0 | 0.141 | 0.143 | 0.142 | | |
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货期
3-5 working days