产品描述
This Rat BCL2L1 ELISA Kit was designed for the quantitative measurement of Rat BCL2L1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.
别名
Bcl2l1; Bclx; Blc2l; Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X
缩写
BCL2L1
Uniprot No
P53563
灵敏度
11.72 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Bcl2-L-1/Bcl-X in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 86-95 | |
| 1:2 | Average % | 102 | |
| Range % | 97-104 | |
| 1:4 | Average % | 91 | |
| Range % | 85-95 | |
| 1:8 | Average % | 97 | |
| Range % | 91-101 | |
回收率
| The recovery of rat Bcl2-L-1/Bcl-X spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 88-94 | |
| EDTA plasma (n=4) | 99 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 3000 | 2.106 | 2.008 | 2.057 | 1.928 | | | 1500 | 1.416 | 1.376 | 1.396 | 1.267 | | | 750 | 0.928 | 0.965 | 0.947 | 0.818 | | | 375 | 0.596 | 0.605 | 0.601 | 0.472 | | | 187.5 | 0.364 | 0.353 | 0.359 | 0.230 | | | 93.75 | 0.293 | 0.284 | 0.289 | 0.160 | | | 46.88 | 0.215 | 0.207 | 0.211 | 0.082 | | | 0 | 0.130 | 0.128 | 0.129 | | |
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货期
3-5 working days