产品描述

别名

Acidic C4 ELISA Kit; Acidic complement C4 ELISA Kit; Basic C4 ELISA Kit; Basic complement C4 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4; Chido form ELISA Kit; C4; Rodgers from ELISA Kit; C4A anaphylatoxin ELISA Kit; C4A ELISA Kit; C4A2 ELISA Kit; C4A3 ELISA Kit; C4A4 ELISA Kit; C4A6 ELISA Kit; C4AD ELISA Kit; C4B ELISA Kit; C4B_2 ELISA Kit; C4B1 ELISA Kit; C4B12 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; C4BD ELISA Kit; C4F ELISA Kit; C4S ELISA Kit; CH ELISA Kit; Chido form of C4 ELISA Kit; CO4 ELISA Kit; CO4A_HUMAN ELISA Kit; Complement C4 A ELISA Kit; Complement C4 B ELISA Kit; Complement C4 gamma chain ELISA Kit; complement C4-A ELISA Kit; complement C4-B ELISA Kit; complement C4-B-like ELISA Kit; complement C4B1a ELISA Kit; Complement component 4A (Rodgers blood group) ELISA Kit; Complement component 4A ELISA Kit; complement component 4B (Chido blood group) ELISA Kit; complement component 4B (Chido blood group); copy 2 ELISA Kit; Complement component 4B (Childo blood group) ELISA Kit; Complement component 4B ELISA Kit; Complement component 4F ELISA Kit; Complement component 4S ELISA Kit; CPAMD2 ELISA Kit; CPAMD3 ELISA Kit; RG ELISA Kit; Rodgers form of C4 ELISA Kit

缩写

C4

Uniprot No

P0C0L4

灵敏度

0.41 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1000		Average %		96
		Range %		93-99
1:2000		Average %		100
		Range %		97-103
1:4000		Average %		89
		Range %		86-92
1:8000		Average %		100
		Range %		97-104

回收率

The recovery of human C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		86		83-90
EDTA plasma (n=4)		95		91-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   300 2.873 2.897 2.885 2.786   100 1.916 1.968 1.942 1.843   33.3 1.281 1.299 1.290 1.191   11.1 0.878 0.898 0.888 0.789   3.7 0.495 0.472 0.484 0.385   1.23 0.195 0.212 0.204 0.105   0.41 0.187 0.192 0.190 0.091   0 0.101 0.097 0.099

货期

3-5 working days