产品描述
This Rat CTSK ELISA Kit was designed for the quantitative measurement of Rat CTSK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Ctsk ELISA Kit; Cathepsin K ELISA Kit; EC 3.4.22.38 ELISA Kit
缩写
CTSK
Uniprot No
O35186
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:2 |
Average % |
99 |
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| Range % |
95-103 |
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| 1:4 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:8 |
Average % |
107 |
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| Range % |
103-110 |
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回收率
| The recovery of rat cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
86 |
83-89 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.801 |
2.765 |
2.783 |
2.682 |
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| 500 |
2.342 |
2.204 |
2.273 |
2.172 |
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| 250 |
1.343 |
1.406 |
1.375 |
1.274 |
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| 125 |
0.679 |
0.701 |
0.690 |
0.589 |
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| 62.5 |
0.399 |
0.405 |
0.402 |
0.301 |
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| 31.2 |
0.251 |
0.242 |
0.247 |
0.146 |
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| 15.6 |
0.166 |
0.164 |
0.165 |
0.064 |
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| 0 |
0.102 |
0.100 |
0.101 |
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货期
3-5 working days