产品描述
This Human CEMP1 ELISA Kit was designed for the quantitative measurement of Human CEMP1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
Cementoblastoma-derived protein 1 ELISA Kit; Cementum protein 1 ELISA Kit; Cementum protein 23 ELISA Kit; CEMP1 ELISA Kit; CEMP1_HUMAN ELISA Kit; CP-23 ELISA Kit; CP23 ELISA Kit
缩写
CEMP1
Uniprot No
Q6PRD7
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 85-95 | |
| 1:2 | Average % | 101 | |
| Range % | 97-105 | |
| 1:4 | Average % | 83 | |
| Range % | 80-86 | |
| 1:8 | Average % | 107 | |
| Range % | 103-110 | |
回收率
| The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-96 | |
| EDTA plasma (n=4) | 91 | 84-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.658 | 2.697 | 2.678 | 2.528 | | | 5 | 2.312 | 2.331 | 2.322 | 2.172 | | | 2.5 | 1.871 | 1.892 | 1.882 | 1.732 | | | 1.25 | 1.312 | 1.365 | 1.339 | 1.189 | | | 0.625 | 0.788 | 0.797 | 0.793 | 0.643 | | | 0.312 | 0.442 | 0.472 | 0.457 | 0.307 | | | 0.156 | 0.281 | 0.272 | 0.277 | 0.127 | | | 0 | 0.151 | 0.149 | 0.150 | | |
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货期
3-5 working days