产品描述
This Human CPA3 ELISA Kit was designed for the quantitative measurement of Human CPA3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
别名
carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit
缩写
CPA3
Uniprot No
P15088
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-92 | |
| 1:2 | Average % | 100 | |
| Range % | 94-103 | |
| 1:4 | Average % | 97 | |
| Range % | 94-100 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 96 | 94-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.477 | 2.453 | 2.465 | 2.366 | | | 10 | 1.993 | 1.924 | 1.959 | 1.860 | | | 5 | 1.280 | 1.271 | 1.276 | 1.177 | | | 2.5 | 0.685 | 0.678 | 0.682 | 0.583 | | | 1.25 | 0.433 | 0.424 | 0.429 | 0.330 | | | 0.625 | 0.286 | 0.269 | 0.278 | 0.179 | | | 0.312 | 0.190 | 0.179 | 0.185 | 0.086 | | | 0 | 0.101 | 0.097 | 0.099 | | |
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货期
3-5 working days