产品描述
This Bovine ADIPOQ ELISA Kit was designed for the quantitative measurement of Bovine ADIPOQ protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.125 μg/mL-50 μg/mL and the sensitivity is 3.125 μg/mL .
别名
ADIPOQ ELISA kit; ACRP30 ELISA kit; APM1Adiponectin ELISA kit; 30 kDa adipocyte complement-related protein ELISA kit; Adipocyte complement-related 30 kDa protein ELISA kit; ACRP30 ELISA kit; Adipocyte ELISA kit; C1q and collagen domain-containing protein ELISA kit; Adipose most abundant gene transcript 1 protein ELISA kit; apM-1 ELISA kit
缩写
ADIPOQ
Uniprot No
Q3Y5Z3
灵敏度
3.125 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 87-97 | |
| 1:2 | Average % | 97 | |
| Range % | 93-104 | |
| 1:4 | Average % | 85 | |
| Range % | 82-91 | |
| 1:8 | Average % | 90 | |
| Range % | 85-97 | |
回收率
| The recovery of bovine ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 98 | 94-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| μg/ml | OD1 | OD2 | Average | | | | 50 | 0.212 | 0.207 | 0.210 | | | | 25 | 0.345 | 0.332 | 0.339 | | | | 12.5 | 0.589 | 0.607 | 0.598 | | | | 6.25 | 1.077 | 1.106 | 1.092 | | | | 3.125 | 2.214 | 2.123 | 2.169 | | |
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货期
3-5 working days