产品描述

This Human BCL6 ELISA Kit was designed for the quantitative measurement of Human BCL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

别名

B cell CLL/lymphoma 6 ELISA Kit; B cell lymphoma 6 protein ELISA Kit; B-cell lymphoma 5 protein ELISA Kit; B-cell lymphoma 6 protein ELISA Kit; BCL 5 ELISA Kit; Bcl 6 ELISA Kit; BCL-5 ELISA Kit; BCL-6 ELISA Kit; BCL5 ELISA Kit; BCL6 ELISA Kit; BCL6_HUMAN ELISA Kit; BCL6A ELISA Kit; cys his2 zinc finger transcription factor ELISA Kit; cys-his2 zinc finger transcription factor ELISA Kit; LAZ 3 ELISA Kit; LAZ 3 protein ELISA Kit; LAZ3 ELISA Kit; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) ELISA Kit; Lymphoma associated zinc finger gene on chromosome 3 ELISA Kit; Protein LAZ-3 ELISA Kit; ZBTB 27 ELISA Kit; ZBTB27 ELISA Kit; Zinc finger and BTB domain containing protein 27 ELISA Kit; Zinc finger and BTB domain-containing protein 27 (ZBTB27) ELISA Kit; Zinc finger and BTB domain-containing protein 27 ELISA Kit; Zinc Finger Protein 51 (ZNF51) ELISA Kit; Zinc finger protein 51 ELISA Kit; zinc finger transcription factor BCL6S ELISA Kit; ZNF 51 ELISA Kit; ZNF51 ELISA Kit

缩写

BCL6

Uniprot No

P41182

灵敏度

4.68 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Bcl6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		98
		Range %		94-103
1:2		Average %		100
		Range %		97-104
1:4		Average %		86
		Range %		83-89
1:8		Average %		98
		Range %		94-103

回收率

The recovery of human Bcl6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		85		81-89
EDTA plasma (n=4)		87		83-90

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1200 2.790 2.701 2.746 2.588 600 2.158 2.032 2.095 1.937 300 1.269 1.304 1.287 1.129 150 0.784 0.811 0.798 0.640 75 0.516 0.531 0.524 0.366 37.5 0.342 0.336 0.339 0.181 18.75 0.246 0.252 0.249 0.091 0 0.157 0.159 0.158

货期

3-5 working days