产品描述
This Rat Aβ1-40 ELISA Kit was designed for the quantitative measurement of Rat Aβ1-40 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL.
别名
amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
缩写
Aβ1-40
灵敏度
0.156 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-98 | |
| 1:2 | Average % | 87 | |
| Range % | 82-91 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 105 | |
| Range % | 100-113 | |
回收率
| The recovery of rat Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 94-102 | |
| EDTA plasma (n=4) | 93 | 87-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.706 | 2.576 | 2.641 | 2.484 | | | 20 | 2.181 | 2.074 | 2.128 | 1.971 | | | 10 | 1.512 | 1.502 | 1.507 | 1.350 | | | 5 | 1.107 | 1.050 | 1.079 | 0.922 | | | 2.5 | 0.624 | 0.669 | 0.647 | 0.490 | | | 1.25 | 0.473 | 0.447 | 0.460 | 0.303 | | | 0.625 | 0.315 | 0.299 | 0.307 | 0.150 | | | 0 | 0.159 | 0.155 | 0.157 | | |
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货期
3-5 working days