产品描述
This Pig Col Ⅲ ELISA Kit was designed for the quantitative measurement of Pig Col Ⅲ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
别名
Collagen Type 3,Col 3,_x000D_
Collagen Type III,Col III
缩写
Col Ⅲ
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
91-103 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-107 |
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| 1:4 |
Average % |
91 |
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| Range % |
85-97 |
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| 1:8 |
Average % |
93 |
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| Range % |
89-95 |
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回收率
| The recovery of pig Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-96 |
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| EDTA plasma (n=4) |
97 |
92-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
1.898 |
1.878 |
1.888 |
1.712 |
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| 200 |
1.279 |
1.256 |
1.268 |
1.092 |
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| 100 |
0.834 |
0.900 |
0.867 |
0.691 |
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| 50 |
0.615 |
0.635 |
0.625 |
0.449 |
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| 25 |
0.411 |
0.435 |
0.423 |
0.247 |
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| 12.5 |
0.331 |
0.355 |
0.343 |
0.167 |
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| 6.25 |
0.298 |
0.287 |
0.293 |
0.117 |
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| 0 |
0.175 |
0.177 |
0.176 |
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货期
3-5 working days