产品描述
This Human CTF1 ELISA Kit was designed for the quantitative measurement of Human CTF1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
Cardiophin 1 ELISA Kit; Cardiophin1 ELISA Kit; Cardiotrophin I ELISA Kit; Cardiotrophin-1 ELISA Kit; Cardiotrophin1 ELISA Kit; CardiotrophinI ELISA Kit; CT 1 ELISA Kit; CT-1 ELISA Kit; CT1 ELISA Kit; CTF 1 ELISA Kit; Ctf1 ELISA Kit; CTF1_HUMAN ELISA Kit
缩写
CTF1
Uniprot No
Q16619
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CT-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 96 | | |
| Range % | 87-105 | | |
| 1:2 | Average % | 95 | | |
| Range % | 90-102 | | |
| 1:4 | Average % | 89 | | |
| Range % | 83-97 | | |
| 1:8 | Average % | 95 | | |
| Range % | 85-102 | | |
回收率
| The recovery of human CT-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 97 | 92-99 | | |
| EDTA plasma (n=4) | 95 | 87-102 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 500 | 2.189 | 2.172 | 2.181 | 2.084 | | | | 250 | 1.617 | 1.639 | 1.628 | 1.531 | | | | 125 | 1.052 | 1.034 | 1.043 | 0.946 | | | | 62.5 | 0.568 | 0.592 | 0.580 | 0.483 | | | | 31.25 | 0.307 | 0.322 | 0.315 | 0.218 | | | | 15.63 | 0.187 | 0.190 | 0.189 | 0.092 | | | | 7.8 | 0.136 | 0.133 | 0.135 | 0.038 | | | | 0 | 0.095 | 0.099 | 0.097 | | | |
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货期
3-5 working days