产品描述
This Rat Col Ⅲ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅲ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
别名
Collagen Type 3,Col 3,_x000D_
Collagen Type III,Col III
缩写
Col Ⅲ
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-104 |
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| 1:4 |
Average % |
95 |
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| Range % |
92-98 |
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| 1:8 |
Average % |
86 |
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| Range % |
80-94 |
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回收率
| The recovery of rat Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
90 |
85-95 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.585 |
2.532 |
2.559 |
2.415 |
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| 5 |
1.795 |
1.719 |
1.757 |
1.613 |
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| 2.5 |
1.083 |
1.021 |
1.052 |
0.908 |
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| 1.25 |
0.657 |
0.705 |
0.681 |
0.537 |
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| 0.625 |
0.418 |
0.450 |
0.434 |
0.290 |
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| 0.312 |
0.299 |
0.314 |
0.307 |
0.163 |
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| 0.156 |
0.242 |
0.229 |
0.236 |
0.092 |
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| 0 |
0.146 |
0.141 |
0.144 |
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货期
3-5 working days