产品描述
This Mouse ANPEP ELISA Kit was designed for the quantitative measurement of Mouse ANPEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39 mU/mL-2500 mU/mL and the sensitivity is 9.7 mU/mL .
别名
Anpep ELISA kit; Lap-1 ELISA kit; Lap1Aminopeptidase N ELISA kit; AP-N ELISA kit; mAPN ELISA kit; EC 3.4.11.2 ELISA kit; Alanyl aminopeptidase ELISA kit; Aminopeptidase M ELISA kit; AP-M ELISA kit; Membrane protein p161 ELISA kit; Microsomal aminopeptidase ELISA kit; CD antigen CD13 ELISA kit
缩写
ANPEP
Uniprot No
P97449
灵敏度
9.7 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
84-97 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-99 |
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| 1:4 |
Average % |
102 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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回收率
| The recovery of mouse ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
82-96 |
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| EDTA plasma (n=4) |
97 |
93-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2500 |
2.500 |
2.400 |
2.450 |
2.285 |
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| 1250 |
1.970 |
1.830 |
1.900 |
1.735 |
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| 625 |
1.119 |
1.075 |
1.097 |
0.932 |
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| 312.5 |
0.706 |
0.717 |
0.712 |
0.547 |
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| 156.25 |
0.447 |
0.458 |
0.453 |
0.288 |
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| 78 |
0.304 |
0.315 |
0.310 |
0.145 |
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| 39 |
0.246 |
0.252 |
0.249 |
0.084 |
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| 0 |
0.164 |
0.165 |
0.165 |
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货期
3-5 working days