产品描述

This Human CAV1 ELISA Kit was designed for the quantitative measurement of Human CAV1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

别名

BSCL3 ELISA Kit; CAV ELISA Kit; CAV1 ELISA Kit; CAV1_HUMAN ELISA Kit; caveolae protein, 22 kD ELISA Kit; caveolin 1 alpha isoform ELISA Kit; caveolin 1 beta isoform ELISA Kit; Caveolin 1 caveolae protein 22kDa ELISA Kit; Caveolin-1 ELISA Kit; Caveolin1 ELISA Kit; cell growth-inhibiting protein 32 ELISA Kit; CGL3 ELISA Kit; LCCNS ELISA Kit; MSTP085 ELISA Kit; OTTHUMP00000025031 ELISA Kit; PPH3 ELISA Kit; VIP 21 ELISA Kit; VIP21 ELISA Kit

缩写

CAV1

Uniprot No

Q03135

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Cav-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		88-98
1:2		Average %		97
		Range %		92-103
1:4		Average %		89
		Range %		85-96
1:8		Average %		93
		Range %		87-96

回收率

The recovery of human Cav-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		95-107
EDTA plasma (n=4)		95		89-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.501 2.552 2.527 2.424 1000 1.863 2.002 1.933 1.830 500 1.254 1.289 1.272 1.169 250 0.821 0.833 0.827 0.724 125 0.498 0.535 0.517 0.414 62.5 0.328 0.346 0.337 0.234 31.25 0.206 0.201 0.204 0.101 0 0.104 0.101 0.103

货期

3-5 working days