产品描述

This Human AKT1 ELISA Kit was designed for the quantitative measurement of Human AKT1 protein in serum, plasma, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

AKT 1 ELISA Kit; AKT ELISA Kit; AKT1 ELISA Kit; AKT1_HUMAN ELISA Kit; C AKT ELISA Kit; cAKT ELISA Kit; MGC99656 ELISA Kit; PKB alpha ELISA Kit; PKB ELISA Kit; PKB-ALPHA ELISA Kit; PRKBA ELISA Kit; Protein Kinase B Alpha ELISA Kit; Protein kinase B ELISA Kit; Proto-oncogene c-Akt ELISA Kit; RAC Alpha ELISA Kit; RAC ELISA Kit; Rac protein kinase alpha ELISA Kit; RAC Serine/Threonine Protein Kinase ELISA Kit; RAC-alpha serine/threonine-protein kinase ELISA Kit; RAC-PK-alpha ELISA Kit; v akt murine thymoma viral oncogene homolog 1 ELISA Kit; vAKT Murine Thymoma Viral Oncogene Homolog 1 ELISA Kit

缩写

AKT1

Uniprot No

P31749

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human AKT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		93-99
1:2		Average %		87
		Range %		82-90
1:4		Average %		101
		Range %		98-104
1:8		Average %		90
		Range %		87-93

回收率

The recovery of human AKT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		85		82-89
EDTA plasma (n=4)		93		90-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml	OD1	OD2	Average	Corrected
20	2.149	2.169	2.159	2.015
10	1.444	1.469	1.457	1.313
5	0.918	0.938	0.928	0.784
2.5	0.577	0.598	0.588	0.444
1.25	0.388	0.399	0.394	0.250
0.625	0.301	0.321	0.311	0.167
0.312	0.253	0.268	0.261	0.117
0	0.143	0.145	0.144

货期

3-5 working days