产品描述
This Dog ANG-Ⅱ ELISA Kit was designed for the quantitative measurement of Dog ANG-Ⅱ protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
别名
angiotension Ⅱ, angiotensin 2, ANG2
缩写
ANG-Ⅱ
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 86-93 | |
| 1:2 | Average % | 98 | |
| Range % | 92-104 | |
| 1:4 | Average % | 88 | |
| Range % | 86-93 | |
| 1:8 | Average % | 89 | |
| Range % | 84-96 | |
回收率
| The recovery of dog ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 88-97 | |
| EDTA plasma (n=4) | 100 | 95-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 1.978 | 2.030 | 2.004 | 1.844 | | | 200 | 1.324 | 1.395 | 1.360 | 1.200 | | | 100 | 0.764 | 0.810 | 0.787 | 0.627 | | | 50 | 0.466 | 0.501 | 0.484 | 0.324 | | | 25 | 0.301 | 0.310 | 0.306 | 0.146 | | | 12.5 | 0.254 | 0.270 | 0.262 | 0.102 | | | 6.25 | 0.204 | 0.211 | 0.208 | 0.048 | | | 0 | 0.158 | 0.162 | 0.160 | | |
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货期
3-5 working days