产品描述
This Human CTSG ELISA Kit was designed for the quantitative measurement of Human CTSG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
CATG ELISA Kit; CATG_HUMAN ELISA Kit; Cathepsin G ELISA Kit; Cathepsin G precursor ELISA Kit; CathepsinG ELISA Kit; CG ELISA Kit; Ctsg ELISA Kit; CTSG protein ELISA Kit; MGC23078 ELISA Kit
缩写
CTSG
Uniprot No
P08311
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human cath-G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 92 | |
| Range % | 80-99 | |
| 1:10 | Average % | 97 | |
| Range % | 91-105 | |
| 1:20 | Average % | 97 | |
| Range % | 88-110 | |
| 1:40 | Average % | 93 | |
| Range % | 85-98 | |
回收率
| The recovery of human cath-G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 96 | 91-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.617 | 2.654 | 2.636 | 2.512 | | | 1000 | 2.205 | 2.254 | 2.230 | 2.106 | | | 500 | 1.636 | 1.705 | 1.671 | 1.547 | | | 250 | 0.943 | 0.989 | 0.966 | 0.842 | | | 125 | 0.509 | 0.554 | 0.532 | 0.408 | | | 62.5 | 0.352 | 0.369 | 0.361 | 0.237 | | | 31.25 | 0.226 | 0.241 | 0.234 | 0.110 | | | 0 | 0.122 | 0.126 | 0.124 | | |
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货期
3-5 working days