产品描述
Apo-B100是一种载脂蛋白,是由APOB基因所编码的一种蛋白质。它是肝细胞中产生的一种大分子蛋白质,其分子量约为512 kDa。与另一种形式的载脂蛋白Apo-B48相比,Apo-B100更为重要,因为它在体内能够结合和运输所有种类的脂质,包括胆固醇、甘油三酯等。
Apo-B100是低密度脂蛋白(LDL)的主要组成部分,它能够通过血液循环将胆固醇和三酰甘油等脂质从肝脏运输到全身细胞中,并在此过程中与受体结合,促进脂质的吸收和利用。
血液中Apo-B100的含量可以反映人体内LDL的数量,因此被用作评估心血管疾病风险的指标之一。高水平的Apo-B100与动脉粥样硬化和心血管疾病的发生风险增加密切相关,因此控制Apo-B100的水平对于心血管健康至关重要。
华美生物所提供的Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测兔血清、血浆、组织匀浆样本中的Apo-B100,其灵敏度为19.5 ng/mL,检测范围为78 ng/mL-5000 ng/mL。
别名
APOBApolipoprotein B ELISA kit; Fragment ELISA kit
缩写
Apo-B100
Uniprot No
P17165
灵敏度
19.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:200 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:400 |
Average % |
104 |
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| Range % |
98-108 |
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| 1:800 |
Average % |
85 |
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| Range % |
80-89 |
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回收率
| The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
93-102 |
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| EDTA plasma (n=4) |
87 |
82-92 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 5000 |
0.092 |
0.091 |
0.092 |
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| 2500 |
0.146 |
0.138 |
0.142 |
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| 1250 |
0.246 |
0.236 |
0.241 |
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| 625 |
0.375 |
0.360 |
0.368 |
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| 312 |
0.524 |
0.539 |
0.532 |
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| 156 |
0.934 |
0.877 |
0.906 |
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| 78 |
1.447 |
1.334 |
1.391 |
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| 0 |
2.432 |
2.562 |
2.497 |
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货期
3-5 working days