产品描述
This Mouse CTNNB1 ELISA Kit was designed for the quantitative measurement of Mouse CTNNB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
Ctnnb1 ELISA Kit; CatnbCatenin beta-1 ELISA Kit; Beta-catenin ELISA Kit
缩写
CTNNB1
Uniprot No
Q02248
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-cat in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-96 | |
| 1:2 | Average % | 99 | |
| Range % | 95-103 | |
| 1:4 | Average % | 101 | |
| Range % | 94-106 | |
| 1:8 | Average % | 95 | |
| Range % | 89-98 | |
回收率
| The recovery of mouse β-cat spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 86-98 | |
| EDTA plasma (n=4) | 99 | 92-106 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.854 | 2.961 | 2.908 | 2.715 | | | 5 | 2.156 | 2.244 | 2.200 | 2.007 | | | 2.5 | 1.433 | 1.498 | 1.466 | 1.273 | | | 1.25 | 0.774 | 0.768 | 0.771 | 0.578 | | | 0.625 | 0.457 | 0.443 | 0.450 | 0.257 | | | 0.312 | 0.324 | 0.339 | 0.332 | 0.139 | | | 0.156 | 0.245 | 0.228 | 0.237 | 0.044 | | | 0 | 0.189 | 0.197 | 0.193 | | |
|
货期
3-5 working days