产品描述
This Mouse ACP5 ELISA Kit was designed for the quantitative measurement of Mouse ACP5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.078 mIU/mL-5 mIU/mL and the sensitivity is 0.02 mIU/mL.
别名
Acp5 ELISA Kit; T5ap ELISA Kit; Trap ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR-AP ELISA Kit; EC 3.1.3.2 ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit
缩写
ACP5
Uniprot No
Q05117
灵敏度
0.02 mIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 100 | |
| Range % | 96-104 | |
| 1:2 | Average % | 98 | |
| Range % | 92-107 | |
| 1:4 | Average % | 93 | |
| Range % | 89-97 | |
| 1:8 | Average % | 95 | |
| Range % | 90-99 | |
回收率
| The recovery of mouse TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 92-102 | |
| EDTA plasma (n=4) | 91 | 87-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| mIU/ml | OD1 | OD2 | Average | Corrected | | | 5 | 2.412 | 2.464 | 2.438 | 2.270 | | | 2.5 | 2.029 | 2.007 | 2.018 | 1.850 | | | 1.25 | 1.655 | 1.564 | 1.610 | 1.442 | | | 0.625 | 1.173 | 1.129 | 1.151 | 0.983 | | | 0.312 | 0.734 | 0.782 | 0.758 | 0.590 | | | 0.156 | 0.491 | 0.456 | 0.474 | 0.306 | | | 0.078 | 0.247 | 0.227 | 0.237 | 0.069 | | | 0 | 0.168 | 0.167 | 0.168 | | |
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货期
3-5 working days