产品描述
This mouse APOA1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse APOA1 in multiple samples, including serum, plasma, cell culture supernates, urine, or tissue homogenates. Antibody specific for APOA1 has been pre-coated onto the microplate. Standards and samples are pipetted into the wells and any APOA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated APOA1 antibody is added to the wells. After washing, avidin conjugated HRP is added to the wells, forming an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound HRP-avidin, the TMB substrate solution is added to the wells, and the color develops into blue. The color changes from blue to yellow after the addition of stop solution into the wells. The color intensity is in proportion to the amount of APOA1 bound in the initial step.
APOA1 is the major component of high-density lipoprotein (HDL) particles in plasma. It is responsible for the transport of excess cholesterol and phospholipids from peripheral tissues to the liver through an initial interaction with the ABCA1 transporter. It has atheroprotective properties. APOA1 helps scavenge fats from white blood cells within artery walls to prevent them from becoming fat overloaded, transforming into foam cells that contribute to progressive atheroma. APOA1 also exerts receptor-independent effects, such as binding to and neutralizing LPS.
别名
Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
缩写
APOA1
Uniprot No
Q00623
灵敏度
7.81 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1000 | Average % | 95 | |
| Range % | 90-100 | |
| 1:2000 | Average % | 89 | |
| Range % | 84-95 | |
| 1:4000 | Average % | 85 | |
| Range % | 80-90 | |
| 1:8000 | Average % | 97 | |
| Range % | 92-101 | |
回收率
| The recovery of mouse Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 93-104 | |
| EDTA plasma (n=4) | 99 | 95-107 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.214 | 2.256 | 2.235 | 2.062 | | | 1000 | 1.856 | 1.890 | 1.873 | 1.700 | | | 500 | 1.461 | 1.400 | 1.431 | 1.258 | | | 250 | 0.964 | 0.999 | 0.982 | 0.809 | | | 125 | 0.689 | 0.672 | 0.681 | 0.508 | | | 62.5 | 0.481 | 0.491 | 0.486 | 0.313 | | | 31.25 | 0.350 | 0.368 | 0.359 | 0.186 | | | 0 | 0.172 | 0.174 | 0.173 | | |
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货期
3-5 working days
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