产品描述

This Human ATG7 ELISA Kit was designed for the quantitative measurement of Human ATG7 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.

别名

1810013K23Rik ELISA Kit; Apg 7 ELISA Kit; APG7 autophagy 7 like ELISA Kit; APG7 autophagy 7-like (S. cerevisiae) ELISA Kit; APG7 like ELISA Kit; APG7; S. cerevisiae; homolog of ELISA Kit; APG7-like ELISA Kit; APG7L ELISA Kit; ATG 7 ELISA Kit; ATG12-activating enzyme E1 ATG7 ELISA Kit; ATG7 ELISA Kit; ATG7 autophagy related 7 homolog (S. cerevisiae) ELISA Kit; ATG7 autophagy related 7 homolog ELISA Kit; ATG7_HUMAN ELISA Kit; Atg7l ELISA Kit; Autophagy 7; S. cerevisiae; homolog of ELISA Kit; Autophagy related protein 7 ELISA Kit; Autophagy-related 7 (yeast) ELISA Kit; Autophagy-related protein 7 ELISA Kit; DKFZp434N0735 ELISA Kit; GSA 7 ELISA Kit; GSA7 ELISA Kit; hAGP7 ELISA Kit; Ubiquitin activating enzyme E1 like protein ELISA Kit; Ubiquitin-activating enzyme E1-like protein ELISA Kit; Ubiquitin-like modifier-activating enzyme ATG7 ELISA Kit

缩写

ATG7

Uniprot No

O95352

灵敏度

7 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ATG7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		82-90
1:2		Average %		101
		Range %		97-104
1:4		Average %		98
		Range %		94-102
1:8		Average %		105
		Range %		101-109

回收率

The recovery of human ATG7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		84		81-89
EDTA plasma (n=4)		101		97-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1800 2.539 2.639 2.589 2.466 900 2.058 2.004 2.031 1.908 450 1.275 1.368 1.322 1.199 225 0.701 0.724 0.713 0.590 112.5 0.401 0.394 0.398 0.275 56 0.253 0.248 0.251 0.128 28 0.190 0.187 0.189 0.066 0 0.124 0.122 0.123

货期

3-5 working days