产品描述
This Pig APOE ELISA Kit was designed for the quantitative measurement of Pig APOE protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.81 ng/mL.
别名
APOEApolipoprotein E ELISA kit; Apo-E ELISA kit
缩写
APOE
Uniprot No
P18650
灵敏度
7.81 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:100 | Average % | 96 | | |
| Range % | 85-105 | | |
| 1:200 | Average % | 90 | | |
| Range % | 89-98 | | |
| 1:400 | Average % | 96 | | |
| Range % | 82-103 | | |
| 1:800 | Average % | 97 | | |
| Range % | 92-99 | | |
回收率
| The recovery of pig Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 97 | 92-105 | | |
| EDTA plasma (n=4) | 95 | 85-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | | | | | 2000 | 0.131 | 0.134 | 0.133 | | | | | 1000 | 0.223 | 0.226 | 0.225 | | | | | 500 | 0.335 | 0.341 | 0.338 | | | | | 250 | 0.562 | 0.553 | 0.558 | | | | | 125 | 0.862 | 0.860 | 0.861 | | | | | 62.5 | 1.192 | 1.214 | 1.203 | | | | | 31.25 | 1.589 | 1.607 | 1.598 | | | | | 0 | 2.417 | 2.395 | 2.406 | | | |
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货期
3-5 working days