产品描述
This Rat AQP2 ELISA Kit was designed for the quantitative measurement of Rat AQP2 protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL .
别名
Aqp2; Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
缩写
AQP2
Uniprot No
P34080
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AQP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 82-93 | |
| 1:2 | Average % | 90 | |
| Range % | 85-95 | |
| 1:4 | Average % | 93 | |
| Range % | 88-97 | |
| 1:8 | Average % | 94 | |
| Range % | 91-98 | |
回收率
| The recovery of rat AQP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 101 | 95-106 | |
| EDTA plasma (n=4) | 94 | 87-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.561 | 2.784 | 2.673 | 2.496 | | | 200 | 1.823 | 1.827 | 1.825 | 1.648 | | | 100 | 1.156 | 1.167 | 1.162 | 0.985 | | | 50 | 0.754 | 0.789 | 0.772 | 0.595 | | | 25 | 0.465 | 0.487 | 0.476 | 0.299 | | | 12.5 | 0.337 | 0.364 | 0.351 | 0.174 | | | 6.25 | 0.264 | 0.275 | 0.270 | 0.093 | | | 0 | 0.175 | 0.179 | 0.177 | | |
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货期
3-5 working days