产品描述

The human TRACP5b ELISA kit (CSB-E08490h) is designed for the quantitative measurement of human TRACP5b protein in serum, plasma, or tissue homogenates. It quantitates human TRACP5b with 0.078 mIU/ml sensitivity and shows excellent specificity for human TRACP5b. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated TRACP5b antibody that recognizes the analyte bound by the immobilized TRACP5b antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of TRACP5b in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the human TRACP5b in the sample.

TRACP5b (ACP5), secreted by osteoclasts, is increased in osteoporosis individuals and is used as a specific and sensitive marker of bone resorption and bone remodeling. After release into circulation, TRACP5b becomes inactive and is degraded into fragments that are removed by the liver. TRACP5b has been applied to diagnose bone diseases such as osteoporosis and bone metastasis and for monitoring antiresorptive treatment. Alatalo et al and Rissanen et al have shown that TRACP5b is a good indicator of osteoclast number in mouse bone marrow-derived osteoclasts and human blood monocyte-derived osteoclasts.

别名

phosphatase ELISA Kit; Acid phosphatase 5 tartrate resistant ELISA Kit; Acid phosphatase 5; tartrate resistant ELISA Kit; ACP5 ELISA Kit; EC 3.1.3.2 ELISA Kit; MGC117378 ELISA Kit; PPA5_HUMAN ELISA Kit; serum band 5 tartrate-resistant acid phosphatase ELISA Kit; SPENCDI ELISA Kit; T5ap ELISA Kit; Tartrate resistant acid ATPase ELISA Kit; Tartrate resistant acid phosphatase type 5 ELISA Kit; Tartrate resistant acid phosphatase type 5 precursor ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR AP ELISA Kit; TR-AP ELISA Kit; TRACP 5 ELISA Kit; TRAP ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit

缩写

ACP5

Uniprot No

P13686

灵敏度

0.078 mIU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		83-92
1:2		Average %		99
		Range %		94-102
1:4		Average %		105
		Range %		100-108
1:8		Average %		95
		Range %		90-98

回收率

The recovery of human TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		92-97
EDTA plasma (n=4)		96		92-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml OD1 OD2 Average Corrected   20 2.484 2.456 2.470 2.357   10 1.918 1.876 1.897 1.784   5 1.262 1.251 1.257 1.144   2.5 0.666 0.715 0.691 0.578   1.25 0.424 0.454 0.439 0.326   0.625 0.312 0.329 0.321 0.208   0.312 0.199 0.208 0.204 0.091   0 0.111 0.115 0.113

货期

3-5 working days