产品描述
阿尔茨海默病相关神经丝蛋白 (AD7C-NTP) 是一种已被确定在阿尔茨海默病发病机制中发挥重要作用的蛋白质。它被发现存在于阿尔茨海默氏症患者的脑组织中,并且还与其他几种神经系统疾病有关。这种蛋白质被认为参与了神经元可塑性的调节,这可能可以解释为什么它与阿尔茨海默氏症的发展有关。研究还表明,AD7C-NTP 可能参与淀粉样斑块的形成,这是这种情况的标志性特征。对 AD7C-NTP 的进一步研究可以帮助我们更好地理解和治疗这种使人衰弱的神经系统疾病。
华美生物所提供的Human alzheimer-associated neuronal thread protein (AD7C-NTP) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的AD7C-NTP,其灵敏度为华美生物所提供的23.43 pg/mL,检测范围为93.75 pg/mL-6000 pg/mL。
缩写
AD7C-NTP
灵敏度
23.43 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of Human AD7C-NTP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
97 |
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| Range % |
93-101 |
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| 1:40 |
Average % |
93 |
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| Range % |
86-97 |
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| 1:80 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:160 |
Average % |
102 |
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| Range % |
98-106 |
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回收率
| The recovery of Human AD7C-NTP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
94-102 |
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| EDTA plasma (n=4) |
87 |
82-93 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 6000 |
2.615 |
2.787 |
2.701 |
2.614 |
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| 3000 |
2.214 |
2.115 |
2.165 |
2.078 |
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| 1500 |
1.441 |
1.386 |
1.414 |
1.327 |
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| 750 |
0.784 |
0.821 |
0.803 |
0.716 |
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| 375 |
0.466 |
0.457 |
0.462 |
0.375 |
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| 187.5 |
0.274 |
0.286 |
0.280 |
0.193 |
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| 93.75 |
0.172 |
0.166 |
0.169 |
0.082 |
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| 0 |
0.086 |
0.087 |
0.087 |
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货期
3-5 working days