产品描述

This Human BMP6 ELISA Kit was designed for the quantitative measurement of Human BMP6 protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

BMP-6 ELISA Kit; Bmp6 ELISA Kit; BMP6_HUMAN ELISA Kit; Bone morphogenetic protein 6 ELISA Kit; Bone Morphogenic Protein 6 ELISA Kit; Decapentaplegic vegetal related ELISA Kit; DVR6 ELISA Kit; HGNC:12686 ELISA Kit; TGFB related vegetal related growth factor ELISA Kit; Transforming growth factor beta ELISA Kit; Vegetal related (TGFB related) cytokine ELISA Kit; Vegetal related growth factor (TGFB related) ELISA Kit; Vg related sequence ELISA Kit; VG-1-R ELISA Kit; VG-1-related protein ELISA Kit; Vg1 related sequence ELISA Kit; VGR ELISA Kit; VGR-1 ELISA Kit; VGR1 ELISA Kit

缩写

BMP6

Uniprot No

P22004

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		88-99
1:2		Average %		95
		Range %		89-102
1:4		Average %		91
		Range %		84-105
1:8		Average %		92
		Range %		86-102

回收率

The recovery of human BMP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		90-101
EDTA plasma (n=4)		92		88-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.280 2.217 2.249 2.154 1000 1.925 1.987 1.956 1.861 500 1.515 1.624 1.570 1.475 250 1.048 1.008 1.028 0.933 125 0.596 0.583 0.590 0.495 62.5 0.374 0.355 0.365 0.270 31.25 0.241 0.252 0.247 0.152 0 0.096 0.093 0.095

货期

3-5 working days