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Anti-P53(wt-p53) Polyclonal Antibody - 产品详情 (solarbio.com)
| 英文名称 | Anti-P53(wt-p53) Polyclonal Antibody |
| 中文名称 | 肿瘤蛋白P53抗体 |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Rat ; Mouse ; Human |
| 预测交叉反应 | Dog;Pig;Horse;Rabbit;Sheep |
| 应用 | ELISA ; FCM ; IF ; ICC ; IHC ; WB |
| 稀释比例 | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg /test. ICC 1:100. IF 1:100-500. |
| 靶标 | P53(wt-p53) |
| 别名 | Widespread p53;Wtp53;Antigen NY-CO-13;Cellular tumor antigen p53;Cys 51 Stop;HGNC11998;LFS1;p53;p53 Cellular Tumor Antigen;p53 Tumor Suppressor;Phosphoprotein p53;TP53;Transformation related protein 53;TRP53;Tumor protein p53;Tumour Protein p53;P53_HUMAN;TP53. |
| 背景说明 | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. |
| Swiss Prot | P04637 |
| Gene ID | 7157 |
| 亚细胞定位 | Cytoplasm Endoplasmic reticulum Mitochondrion matrix Nucleus PML body |
| 免疫原 | A synthetic peptide of human P53(wt-p53) |
| Public Immunogen Range | 301-393/393aa |
| 理论分子量 | 23/30/34/38/44kDa |
| 纯化方法 | Affinity purification |
| 储存液 | Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
| 规格 | 50ul ; 100ul |
| 单位 | 支 |
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Western blot analysis with P53(wt-p53) antibody diluted at 1:300;Lane: Mouse Liver Rat Liver.
Immunohistochemistry of paraffin-embedded Rat brain with P53(wt-p53) antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded rat brain with P53 antibody diluted at 1:200.
Immunofluorescence analysis of A549 cell using P53 antibody diluted at 1:100.
相关标记
BiotinCy3Cy3.5Cy5Cy5.5Cy7FITCHRPRBITCSAlexa Fluor 350SAlexa Fluor 405SAlexa Fluor 488SAlexa Fluor 532SAlexa Fluor 555SAlexa Fluor 568SAlexa Fluor 594SAlexa Fluor 633SAlexa Fluor 640SAlexa Fluor 647SAlexa Fluor 660SAlexa Fluor 680SAlexa Fluor 750
