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Anti-PD-L1/CD274 Polyclonal Antibody - 产品详情 (solarbio.com)
| 英文名称 | Anti-PD-L1/CD274 Polyclonal Antibody |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Rat ; Mouse ; Human |
| 应用 | ELISA ; FCM ; IP ; IF ; IHC ; WB |
| 稀释比例 | WB 1:500-1000. IHC 1:300-1200.IF 1:10-100.IP 0.5-4.0 ug for IP and 1:500-2000 for WB |
| 靶标 | PD-L1/CD274 |
| 背景说明 | Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, it is predicted to be 27-30 kDa after signal peptide cleavage (PMID: 25609200; 17076679). The apparent molecular weight has also been reported as 45-70 kDa, major glycosylated form of 45-50 kDa and multiple post-translational modifications form of 65-70 kDa (PMID: 18760278; 16493058). |
| Swiss Prot | Q9NZQ7 |
| Gene ID | 29126 |
| 亚细胞定位 | Cell membrane |
| 免疫原 | Recombinant protein of human PD-L1/CD274 |
| Public Immunogen Range | 25-241aa |
| Public Immunogen Range | 25-241aa |
| 理论分子量 | 33kDa |
| 纯化方法 | Affinity purification |
| 储存液 | Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
| 规格 | 50ul ; 100ul |
| 单位 | 支 |
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Western blot analysis with PD-L1/CD274 antibody diluted at 1:600;Lane: Human Placenta
Immunohistochemistry of paraffin-embedded Human tonsillitis tissue with PD-L1/CD274 antibody diluted at 1:600
Immunofluorescence analysis of HEK293 cells using PD-L1/CD274 antibody diluted at 1:25.
相关标记
BiotinCy3Cy3.5Cy5Cy5.5Cy7FITCHRPRBITCSAlexa Fluor 350SAlexa Fluor 405SAlexa Fluor 488SAlexa Fluor 532SAlexa Fluor 555SAlexa Fluor 568SAlexa Fluor 594SAlexa Fluor 633SAlexa Fluor 640SAlexa Fluor 647SAlexa Fluor 660SAlexa Fluor 680SAlexa Fluor 750
