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检测原理
Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠IL-6单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠IL-6会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠IL-6抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠IL-6发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠IL-6，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠IL-6浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠IL-6的浓度。
背景介绍
白细胞介素-6（IL-6）是由纤维母细胞、单核/巨噬细胞、T淋巴细 胞、B淋巴细胞、上皮细胞、角质细胞、以及多种瘤细胞所产生。IL-1、 TNF-α、PDGF、病毒感染、双链RNA及cAMP等，均可诱导正常细胞 产生白介素6。白介素6能够刺激参与免疫反应的细胞增殖、分化并提 高其功能。IL-6由2条糖蛋白链组成；1条为α链，分子量80KD；另1 条为β链，分子量130KD。α链缺少胞内区，只能以低亲合性与IL-6结 合，所形成的复合物迅即与高亲和性的β链结合，通过β链向细胞内传递 信息。IL-6主要有以下生物特性：诱导B细胞分化；支持浆细胞瘤和骨 髓瘤增生；诱导IL-2和IL-2受体表达；诱导单核细胞分化；诱导CTL； 增强NK细胞活性；诱导急性期反应分子并刺激肝细胞；诱导神经元分 化；诱导肾小球膜细胞生长；诱导角质化细胞生长。
注意事项
Considerations
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
论文引用
Paper Citation
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折叠
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