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In Vivo
Mice(240 C57BL/6N mice, aged 10 weeks,weight ranging between 19 and 20 g, 100 μg/mL gentamicin)
Specifically, the ABX mice underwent a disinfection step by being briefly immersed in Lugol's iodine solution at 39°C for 3 s,23 followed by consumption of sterilized water containing 100 μg/mL gentamicin (IG0770, Solarbio Science & Technology Co., Ltd.), 2.5 mg/mL sulbenicillin (Solarbio, YZ130345), ephalosporin (Solarbio, IC0870), and 30 μg/mL amphotericin B (Solarbio, IA0321), as previously reported.
文献来源:Zhen Y, Wang Y, He F, Chen Y, Hu L, Ge L, Wang Y, Wei W, Rahmat A, Loor JJ, Wang M. Homeostatic crosstalk among gut microbiome, hypothalamic and hepatic circadian clock oscillations, immunity and metabolism in response to different light-dark cycles: A multiomics study. J Pineal Res. 2023 Sep;75(2):e12892. doi: 10.1111/jpi.12892. Epub 2023 Jul 18. PMID: 37317652.