使用本产品的案例（仅供参考）In VitroCell（Adipose-derived stem cells/ADSCs; 0.3 M ~1.25 M Trehalose）Cryoprotective agents(CPAs) preparationFor the CPA preparation, we used the following methods: (i) trehalose (Tre) group: trehalose powder (Solarbio, China) was diluted with phosphate buffer saline (PBS) for the powder reconstitution according to the required concentrations (0.3 M Tre, 0.6 M Tre, 1.0M Tre, and 1.25 M Tre) and filtered using a 0.22-μm filter;(ii) glycerol (Gly) group: glycerol was diluted with PBS to yield the required concentration (10% Gly, 20% Gly, and 30% Gly);(iii) trehalose and glycerin group: 1.0 M Tre + 20% Gly, as previously described, trehalose powder was diluted with PBS and then diluted with glycerol according to our needs;(iv) 10% DMSO + 90% FBS as a positive control; and (v) control group: only PBS without trehalose and glyceron as a negative control. At least 30 ml of the CPA (30 vials) was preserved per group.来源文献：Zhang TY, Tan PC, Xie Y, Zhang XJ, Zhang PQ, Gao YM, Zhou SB, Li QF. The combination of trehalose and glycerol: an effective and non-toxic recipe for cryopreservation of human adipose-derived stem cells. Stem Cell Res Ther. 2020 Oct 31;11(1):460. doi: 10.1186/s13287-020-01969-0. PMID: 33129347; PMCID: PMC7602354.