一种重要的营养素和氧化还原剂，在骨盐代谢及骨形成中具有重要作用。在体外，它能增加钙盐的沉积，促进矿化结节的形成，并通过间充质细胞间接调节破骨细胞的分化。维生素C能直接抑制核因子κB受体活化子配体（RANKL）诱导的破骨细胞形成 。 使用本产品的应用案例（仅供参考）In VitroCell（Osteogenic/成骨诱导；50 mg/l ascorbic acid）The osteogenic medium was the complete culture medium supplementedwith 10 nM dexamethasone(Solarbio),10 mM β-glycerophosphate (Solarbio),and 50 mg/l ascorbic acid (Solarbio).数据来源文献：Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821.Cell（Osteogenic/成骨诱导；50 mg/l ascorbic acid）Osteogenic induced medium contained complete culture medium supplementedwith 100 nM dexamethasone (Solarbio, Beijing, China), 10 mM β-glycerophosphate (Solarbio) and 50 mg/l ascorbic acid (Solarbio). The medium was replaced every other day. Alizarin Red–positive mineralized matrix was used to evaluate osteogenic capacity by Alizarin red (AR) staining.数据来源文献：Zhao Y, Liu H, Xi X, Chen S, Liu D. TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 2020 Dec 1;397(1):112336. doi: 10.1016/j.yexcr.2020.112336. Epub 2020 Oct 19. PMID: 33091421.Cell（Osteogenic/成骨诱导；MC3T3-E1 cells；50μg/mL L-ascorbic acid）To induce osteoblast differentiation, the isolated mouseBMSCs (2 × 105 cells/well) were treated with DMSO orS6 (8μM) in a modified α-MEM containing 10% of FBS,50μg/ml L-ascorbic acid, 1mM dexamethasone and 1Mβ-glycerophosphate in 12-well plates. 文献来源：Sun L, Zhang Y, Chen G, Ji Y, Ma Q, Qiao X, Wu S, Zhou L, Bu J, Zhu X, Zhang X, Jiang X, Liu C, Li X, Liu Y, Yang Y, Liu C. Targeting SOST using a small-molecule compound retards breast cancer bone metastasis. Mol Cancer. 2022 Dec 29;21(1):228. doi: 10.1186/s12943-022-01697-4. PMID: 36581888; PMCID: PMC9798707.In VivoRat（雄性新生SD Rat；100 mg vitamin C/kg bw/d；灌胃；30d）Forty-five male neonatal SD rats were randomly divided into five groups (n=9 each): corn oil control group, 500 mg DEHP/kg of body weight per day (bw/d) group, 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d group, 500 mg DEHP/kg bw/d+100 mg vitamin C/kg bw/d group, and 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d+100 mg vitamin C/kg bw/d group. DEHP and vitamin E (Solarbio, China) were dissolved in corn oil, and vitamin C (Solarbio,China) was dissolved in distilled water. From postnatal day (PND) 1 to 30, the male rats were administered corn oil, DEHP, vitamin C, and/or vitamin E by gavage each day according to their group. On PND 36, six rats of each groups were sacrificed and their testes were immediately isolated. The rest of rats were fed with routine feed until PND 90.来源文献：Tang X, Wu S, Shen L, Wei Y, Cao X, Wang Y, Long C, Zhou Y, Li D, Huang F, Liu B, Wei G. Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats. Environ Toxicol. 2018 Jul;33(7):720-728. doi: 10.1002/tox.22559. Epub 2018 Apr 16. PMID: 29663635.