图文详情
使用本产品的案例(仅供参考)In VitroCell(the adipogenic induced cells;;1 μM Nile red ;5 min,RT)As to Nile red fluorescent staining, the adipogenic induced cells were washed three times and stained with 1 μM Nile red (Solarbio, Beijing, China) with Hoechst 33342 (Solarbio, Beijing, China) for 5 min in room temperature.来源文献:He F, Yang C, Liu H, Wang J. Changes in the mechanical properties of human mesenchymal stem cells during differentiation. R Soc Open Sci. 2023 Jan 4;10(1):220607. doi: 10.1098/rsos.220607. PMID: 36636310; PMCID: PMC9810430.HBBP(50 μL Nile red (0.01%, w/v)solutions were added to 1 mL of emulsion)The microstructure of the sample was observed using a confocal laser scanning microscope with a 40×objective lens in the fluorescence mode . To dye the oil phase and protein of the emulsion, 50 μL Nile blue (0.01%, w/v) and Nile red (0.01%, w/v)solutions were added to 1 mL of emulsion,;respectively. After uniform mixing and dark staining for 20 min, Nile Blue and Nile Red images were obtained at excitation wavelengths of 488 nm and 633 nm, respectively.来源文献:Zhu Y, Hong N, Zhao L, Liu S, Zhang J, Li M, Ma Y, Liang D, Zhao G. Effect of Molecular Weight on the Structural and Emulsifying Characteristics of Bovine Bone Protein Hydrolysate. Foods. 2023 Dec 18;12(24):4515. doi: 10.3390/foods12244515. PMID: 38137319; PMCID: PMC10743285.