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The spike protein of bat coronavirus Anlong103, a SARS-CoV-2–related sarbecovirus identified in bats from China’s Guizhou province, mediates viral entry by facilitating receptor attachment and membrane fusion. While closely related to other bat coronaviruses, Anlong103’s spike shows distinct structural features that may limit human ACE2 binding, yet retains conserved antigenic sites. These characteristics make it valuable for studying cross-species transmission potential and coronavirus evolution in wildlife reservoirs.