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CD8 alpha is a critical surface protein on cytotoxic T cells. It acts as a co-receptor for the T-cell receptor (TCR), binding to MHC class I molecules to stabilize antigen recognition and enhance T-cell activation. It is predominantly expressed on the surface of cytotoxic T lymphocytes (CTLs) and a subset of natural killer (NK) cells. Anti-CD8 antibody are usually used to modify LNP in in vivo CAR, CD8 alpha protein could be used to detect anti-CD8 antibody density.