T7 High Yield RNA Synthesis Kit for Co-transcription optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA with cap1 efficiently by using T7 RNA polymerase, linear double-stranded DNA with the T7 promoter sequence as the template, NTPs, including GAG, as the substrates to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the system to prepare biotin or dye-labeled RNA. The kit can synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template. The kit uses the co-transcriptional capping strategy to produce the Cap1 RNA. Also modified UTP is substituted with N1-Me-Pseudo UTP to decrease the immunogenicity.

The RNA synthesized by in vitro transcription can be used for various downstream applications, such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.

Contents

Applications

In vitro RNA synthesis.

Shipping and Storage

Dry ice transportation. Store at -15℃ ~ -20℃, valid for two years.

Notes:

1. Be careful not to mix RNase in the reaction system.

2. Experiment equipment (such as: pipette tip, product tube, etc.) should strictly use RNase-free products.

3. For your safety and health, please wear lab coats and disposable gloves.

4. For research use only!

Synthesis principle

Figure 1: In vitro RNA co-transcription process