Species

Human

Protein Construction

IL-4R (Met26-His232),Accession # NP_000409

Tag

C-terminal hFc tag

Expression System

CHO

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

65-70 kDa, reducing conditions

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

<1 EU per 1 mg of the protein by the LAL method

Biological Activity

Immobilized Recombinant Human IL-4R-hFc (Catalog # KB-002) at 2 μg/mL (50 μL/well) can bind Anti-IL-4R Antibody (Dupilumab biosimilar)(Catalog # KA-026), the EC50 is 14.72 ng/mL.

Formulation

Lyophilized from a 0.22μm filtered solution in PBS,5% trehalose.

Reconstitution

Briefly centrifuge the vial before opening to bring the contents to the bottom. Reconstitute at 100 μg/mL in sterile deionized water or PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage&Stability

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• 12 months from date of receipt,at lower than -70°C as supplied.
• 3 months,-20°C under sterile conditions after reconstitution.
• 1 month,2 to 8°C under sterile conditions after reconstitution.

Background

Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. The membrane-bound form of CD124 can be hydrolyzed to a soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.