Species

Human             

Protein Construction

Her2/ErbB2  (Thr23-Thr652),Accession # NP_004439

Tag

C-terminal hFc tag 

Expression System

CHO

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

96 kDa (monomer)  

SDS-PAGE

110-125 kDa, reducing conditions

Purity

> 95% as analyzed by SDS-PAGE   

Endotoxin Level

<1 EU per 1 mg of the protein by the LAL method

Biological Activity

Immobilized Recombinant Human Her2-hFc  (Catalog # KB-001) at 2 μg/mL (50 μL/well) can bind Anti-Her2/ErbB2 Antibody (Trastuzumab biosimilar)(Catalog # KA-001), the EC50 is 23.8 ng/mL.             

Formulation

Lyophilized from a 0.22μm filtered solution in PBS,5% trehalose.

Reconstitution

Briefly centrifuge the vial before opening to bring the contents to the bottom. Reconstitute at 100 μg/mL in sterile deionized water or PBS.                  

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.                   

Storage&Stability

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.             

• 12 months from date of receipt,at lower than -70°C as supplied.
• 3 months,-20°C under sterile conditions after reconstitution.
• 1 month,2 to 8°C under sterile conditions after reconstitution.

Background

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.