Recombinant Human Noggin

Catalog Number: Y03301

Characteristics:

Source

Chinese Hamster Ovary cell line

Description

Human Noggin (Gln28-Cys232)

Accession # Q13253

Predicted molecular mass

23 kDa

Specification:

Appearance

White powder, Colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Activity

Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED₅₀ for this effect is 2.0-3.0 ng/ml of Noggin.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing  PBS, 5% mannitol and 0.01% Tween 80, pH 7.4

Size

50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.