Recombinant Human TGF-β1

Catalog Number: Y04201N

Characteristics:

Source

Chinese Hamster Ovary cell line

Description

Human TGF-β1 (Ala279-Ser390)

Accession # P01137

Predicted molecular mass

12.8 kDa(monomer)

Specification:

Appearance

White powder, Colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Activity

Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED₅₀ for this effect is ≤0.1ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA,

30% ACN, 5% mannitol, pH 2.5

Size

50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution

It is recommended to redissolve in 4mM HCL.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.