Recombinant Human Fetuin A

Catalog Number: Y07501

Characteristics:

Source Chinese Hamster Ovary cell line

Description Human Fetuin A (A-Chain: A19-L300; B-Chain: T341-V367)

Accession # P02765

Predicted molecular mass 32.9 KDa

Specification:

Appearance White powder, Colorless clear liquid after reconstitution

Purity ≥95%, by SDS-PAGE (under reducing (R) condition, visualized by Coomassie staining)

Endotoxin ≤0.5 EU/mg by the LAL method

Activity Measured in a cell proliferation assay using B16-F1 cells. Human Fetuin A (Cat. No. Y07501) stimulates adhesion of B16-F1 cells. The ED50 for this effect is 50-200 μg/mL.

Optimal concentration depends on cell type as well as the application or research objectives.

Formulation Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, pH 7.4

Size 100 mg/vial, 1g/vial, 10g/vial, 100g/vial

Handling and Storage:

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.