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收藏小鼠单克隆抗体是由激活的B淋巴细胞与骨髓瘤细胞融合而成的单克隆杂交瘤细胞产生的。鼠单克隆抗体可针对单一抗原决定簇,特异性高,传代稳定,可大规模重复生产,在基础研究、医学诊断、肿瘤靶向治疗、放射免疫显影等多个领域有着广泛的应用。基于杂交瘤技术,义翘神州可制备高灵敏度、高特异性的鼠单克隆抗体,还可以按客户要求提供灵活的定制化方案,旨在为客户提供高品质精品抗体。 我们的定制鼠单克隆抗体生产服务可根据您最终应用要求,制定个性化免疫、筛选及检测方案,以满足您的验证要求。义翘神州拥有大规模杂交瘤抗体生产经验,可提供从毫克到克的抗体生产。另外,如果您的项目时间很紧张,您还可以选择我们的快速鼠单克隆抗体制备服务。

周期: 4-6个月

注意: 客户可提供抗原或选择义翘神州抗原制备服务。
附加服务: 杂交瘤细胞测序服务、杂交瘤细胞培养抗体生产服务

义翘神州建立了高通量鼠单克隆抗体制备平台,通过对免疫方案、融合方案、筛选方案、高通量抗体生产纯化方案的优化,建立了一整套鼠单克隆抗体高通量研发方案。鼠单抗均经过 ELISA,WB,IHC,FACS 等多种平台验证,多细胞多组织验证。
多细胞、多组织验证
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| Fig 1. Immunochemical staining of human target D with mouse mAb. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen. | Fig 2. Immunochemical staining of human target D with mouse monoclonal antibody. |
多应用验证
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| Fig 1. Confocal immunofluorescence analysis of human target D in MCF7 cells. Positive staining was localized to lysosome membrane. | Fig 2. Flow cytometric analysis of human target D on Jurkat cells. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
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| Lane A: Jurkat Whole Cell Lysate. Anti-target D mouse Mab; goat Anti-mouse IgG H&L (Dylight800). | Fig 4. Lane A: Hela Whole Cell Lysate; Lane B: Jurkat Whole Cell Lysate; Lane C: Daudi Whole Cell Lysate. Anti-target D mouse Mab; Dylight 800-labeled antibody to Mouse IgG (H+L). |