Oncostatin M/OSM Protein, Human, Recombinant, HPLC-verified: Product Information
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Endotoxin<5 EU per mg protein
ActivityMeasured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.3-1.2 ng/mL.The specific activity of recombinant human OSM is >30,000 Units/μg, which is calibrated against the human OSM WHO International Standard (NIBSC code: 93/564).
Protein ConstructionA DNA sequence encoding the human OSM (NP_065391.1) (Met1-Arg221) was expressed.
Expressed HostHEK293 Cells
SpeciesHuman
Predicted N TerminalAla 26
Molecule MassThe recombinant human OSM consists of 196 amino acids and predicts a molecular mass of 22.16 kDa. As a result of glycosylation, it migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
FormulationSupplied as sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
ShippingLiquid. It is shipped out with blue ice.
Stability & StorageSamples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
ReconstitutionA hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.






Oncostatin M/OSM 背景信息
Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeⅠand typeⅡ both of which share the similarity of containing protein gp130 and takes part in many bio metabolism processes including liver development, hematopoiesis, inflammation, bone formation, and destruction and possibly CNS development. Oncostatin M (OSM) was previously identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6, and G-CSF, can inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degrees but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-terminal most 31 amino acids produces the fully active 196 residue form.
全称
oncostatin M