图文详情
重组人Ku70 & Ku80 Heterodimer蛋白(产品说明)
蛋白名称:Human Ku70 & Ku80 Heterodimer Protein (His Tag)
蛋白构建:A DNA sequence encoding the Human XRCC5 (P13010) (Met1-Ile732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1 ; A DNA sequence encoding the Human XRCC6 (P12956) (Met1-Asp609) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the Human Ku70 & Ku80 Heterodimer was purified. The purified protein was biotinylated in vitro.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Met 1 & Met 1
蛋白分子量:The recombinant Human Ku70 & Ku80 Heterodimer comprises 1367 (758+609) amino acids and has a calculated molecular mass of 155.02 (85.17+69.85) kDa. It migrates as an approximately 90.9 and 69.5 KDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 153.3 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:P13010 & P12956
蛋白氨基酸序列:Met1-Ile732 & Met1-Asp609