图文详情
重组人 VCAM-1 / CD106 蛋白 (ECD, Fc标签)(产品说明)
蛋白名称:Human VCAM-1 / CD106 Protein (ECD, Fc Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Pro 697) of human VCAM1 (NP_001069.1) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates (10 μg/mL, 100 μL/well) approximately > 70% cells will adhere after 1 hour of incubation at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 25
蛋白分子量:The recombinant human VCAM1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 911 amino acids and has a predicted molecular mass of 100.8 kDa. As a result of glycosylation, the rh VCAM1/Fc monomer migrates as an approximately 116.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001069.1
蛋白氨基酸序列:Met1-Pro697
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.