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APRIL/TNFSF13重组蛋白|Recombinant Human APRIL/TNFSF13 Protein (His & AVI Tag), Biotinylated(产品说明)
蛋白名称:Human APRIL/TNFSF13 Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human TNFSF13 (O75888-1) (Gln111-Leu250) was expressed with a N-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. This construct has a trimer design.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白活性:1.Immobilized Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, Fc tag) (Cat: 10620-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human APRIL/TNFSF13 Protein (His & AVI Tag), Biotinylated (Cat: 10610-H44H1-B), the EC50 is 2-6 ng/mL.
2.Loaded Biotinylated Recombinant Human APRIL/TNFSF13 Protein, His & AVI Tag (Cat. No. 10610-H44H1-B) on SA Biosensor, can bind Recombinant Human TACI Protein, hFc Tag (Cat. No. 29965-H02H) with an affinity constant of 39.200 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gln 111
蛋白分子量:The recombinant Human TNFSF13 has a predicted molecular mass of 53.11 kDa. It migrates as approximately 53.3 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 64.9 kDa verified by SEC-MALS.
蛋白NP号:O75888-1
蛋白氨基酸序列:Gln111-Leu250